pdd cas9 (Addgene inc)
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Addgene inc
pdd cas9
Pdd Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdd+cas9/DD-Cas9+with+filler+sequence+and+Venus+(EDCPV)+(Plasmid+%2390085)/pmc09805690-188-52-53
Average 93 stars, based on 14 article reviews
Pdd Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdd+cas9/DD-Cas9+with+filler+sequence+and+Venus+(EDCPV)+(Plasmid+%2390085)/pmc09805690-188-52-53
Average 93 stars, based on 14 article reviews
pdd cas9 - by Bioz Stars,
2026-09
93/100 stars
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Mutagenesis:Article Title: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells Article Snippet: Full length SHOX was amplified from 293T cDNA and cloned into pHAGE-EF1a-IRES-zsGreen (Addgene #114008) using NotI;BamHI sites, creating pSHOX-GFP. .. Mutant MSC and 293T cells were generated using 2-part lentiviral pCas9-Puro and pgRNA-GFP/Cherry system. pCas9-Puro were generated by Gibson assembly using PCR products of primers CP-1 on PX458 (Addgene #48138) and CP-2 on pLenti-TRE3G-BE3RA-PGK-Puro (Addgene #110846) and backbone of ClaI;NotI cut pHAGE-EF1a-IRES-zsGreen. pgRNA-GFP was constructed by inserting a gRNA cassette cloned from Generated:Article Title: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells Article Snippet: Full length SHOX was amplified from 293T cDNA and cloned into pHAGE-EF1a-IRES-zsGreen (Addgene #114008) using NotI;BamHI sites, creating pSHOX-GFP. .. Mutant MSC and 293T cells were generated using 2-part lentiviral pCas9-Puro and pgRNA-GFP/Cherry system. pCas9-Puro were generated by Gibson assembly using PCR products of primers CP-1 on PX458 (Addgene #48138) and CP-2 on pLenti-TRE3G-BE3RA-PGK-Puro (Addgene #110846) and backbone of ClaI;NotI cut pHAGE-EF1a-IRES-zsGreen. pgRNA-GFP was constructed by inserting a gRNA cassette cloned from Polymerase Chain Reaction:Article Title: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells Article Snippet: Full length SHOX was amplified from 293T cDNA and cloned into pHAGE-EF1a-IRES-zsGreen (Addgene #114008) using NotI;BamHI sites, creating pSHOX-GFP. .. Mutant MSC and 293T cells were generated using 2-part lentiviral pCas9-Puro and pgRNA-GFP/Cherry system. pCas9-Puro were generated by Gibson assembly using PCR products of primers CP-1 on PX458 (Addgene #48138) and CP-2 on pLenti-TRE3G-BE3RA-PGK-Puro (Addgene #110846) and backbone of ClaI;NotI cut pHAGE-EF1a-IRES-zsGreen. pgRNA-GFP was constructed by inserting a gRNA cassette cloned from Construct:Article Title: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells Article Snippet: Full length SHOX was amplified from 293T cDNA and cloned into pHAGE-EF1a-IRES-zsGreen (Addgene #114008) using NotI;BamHI sites, creating pSHOX-GFP. .. Mutant MSC and 293T cells were generated using 2-part lentiviral pCas9-Puro and pgRNA-GFP/Cherry system. pCas9-Puro were generated by Gibson assembly using PCR products of primers CP-1 on PX458 (Addgene #48138) and CP-2 on pLenti-TRE3G-BE3RA-PGK-Puro (Addgene #110846) and backbone of ClaI;NotI cut pHAGE-EF1a-IRES-zsGreen. pgRNA-GFP was constructed by inserting a gRNA cassette cloned from Article Title: Rescue of Recombinant Adenoviruses by CRISPR/Cas-Mediated in vivo Terminal Resolution Article Snippet: .. The expression cassette coding for the destabilized, human codon-optimized Cas9 fusion protein (DD-Cas9) was constructed on the basis of Clone Assay:Article Title: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells Article Snippet: Full length SHOX was amplified from 293T cDNA and cloned into pHAGE-EF1a-IRES-zsGreen (Addgene #114008) using NotI;BamHI sites, creating pSHOX-GFP. .. Mutant MSC and 293T cells were generated using 2-part lentiviral pCas9-Puro and pgRNA-GFP/Cherry system. pCas9-Puro were generated by Gibson assembly using PCR products of primers CP-1 on PX458 (Addgene #48138) and CP-2 on pLenti-TRE3G-BE3RA-PGK-Puro (Addgene #110846) and backbone of ClaI;NotI cut pHAGE-EF1a-IRES-zsGreen. pgRNA-GFP was constructed by inserting a gRNA cassette cloned from Cloning:Article Title: Targeting G-quadruplex for rescuing impaired chondrogenesis in WRN-deficient stem cells Article Snippet: Full length SHOX was amplified from 293T cDNA and cloned into pHAGE-EF1a-IRES-zsGreen (Addgene #114008) using NotI;BamHI sites, creating pSHOX-GFP. .. Mutant MSC and 293T cells were generated using 2-part lentiviral pCas9-Puro and pgRNA-GFP/Cherry system. pCas9-Puro were generated by Gibson assembly using PCR products of primers CP-1 on PX458 (Addgene #48138) and CP-2 on pLenti-TRE3G-BE3RA-PGK-Puro (Addgene #110846) and backbone of ClaI;NotI cut pHAGE-EF1a-IRES-zsGreen. pgRNA-GFP was constructed by inserting a gRNA cassette cloned from Expressing:Article Title: Rescue of Recombinant Adenoviruses by CRISPR/Cas-Mediated in vivo Terminal Resolution Article Snippet: .. The expression cassette coding for the destabilized, human codon-optimized Cas9 fusion protein (DD-Cas9) was constructed on the basis of Plasmid Preparation:Article Title: Rescue of Recombinant Adenoviruses by CRISPR/Cas-Mediated in vivo Terminal Resolution Article Snippet: .. The expression cassette coding for the destabilized, human codon-optimized Cas9 fusion protein (DD-Cas9) was constructed on the basis of Sequencing:Article Title: Rescue of Recombinant Adenoviruses by CRISPR/Cas-Mediated in vivo Terminal Resolution Article Snippet: .. The expression cassette coding for the destabilized, human codon-optimized Cas9 fusion protein (DD-Cas9) was constructed on the basis of |